Wednesday, March 4, 2015

How to Use Volumetric Pipettes

Instructions

Place the tip of the pipette in the solution to be measured. Hold the pipette bulb in your other hand, compress the bulb, then place the opening of the bulb over the top of the pipette and release it. The expansion of the rubber bulb will create suction through the pipette that will draw the liquid into it.

Remove the pipette from the solution, hold it over a sink or drain. Gradually release the pressure of your finger until the liquid level in the pipette begins to drop. Slowly bring the liquid level in the pipette to the mark that indicates the proper fill level. The liquid in the stem will form a U shape called a "miniscus." The bottom of the miniscus represents the volume level. When the miniscus reaches the mark on the stem, reapply pressure with your finger to keep the liquid level from falling below the mark.

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